cell lines panc 10 05  (ATCC)

Journal: Cancer Reports

Article Title: Senolysis of gemcitabine‐induced senescent human pancreatic cancer cells

doi: 10.1002/cnr2.2075

Figure Lengend Snippet: Effects of gemcitabine (GEM) on four human pancreatic cancer cell lines. Four pancreatic cancer lines were cultured with the indicated doses of GEM for 2 days (A) and 4 days (B), and cell viability was determined using a Cell Counting Kit‐8 (CCK) assay. Data are means ± standard deviation (SD). (C) Four cancer cell lines were cultured in the presence of GEM (0.0125 μM) for 2 and 4 days and photographed. Magnification ×20; Scale bar 100 μm.

Article Snippet: Three human pancreatic cancer cell lines (AsPC‐1, CFPAC‐1, and PANC10.05) were purchased from the ATCC.

Techniques: Cell Culture, Cell Counting, Standard Deviation

Journal: Cancer Reports

Article Title: Senolysis of gemcitabine‐induced senescent human pancreatic cancer cells

doi: 10.1002/cnr2.2075

Figure Lengend Snippet: Gemcitabine (GEM) induces senescent features in PANC‐1 and AsPC‐1 cells. (A) Four pancreatic cancer cell lines were cultured with GEM (0.0125 μM) for 48 h. The levels of the p21 and p16 proteins were determined via immunoblotting. β‐Actin was used as the loading control. (B) PANC‐1 and AsPC‐1 cells were cultured with GEM (0.0125 μM). Total RNAs were extracted and real‐time polymerase chain reaction (qPCR) was performed. (C) Four cell lines were cultured in 12‐well plates with GEM (PANC‐1 and AsPC‐1, 0.0125 μM; CFPAC‐1, 0.0008 μM; PANC10.05, 0.0032 μM) for 3 days. Using the Cellular Senescence Detection Kit, SA‐β‐gal was stained. Forward side scattering (FSC) and the SA SPiDER‐β‐gal levels were measured by flow cytometry. The red and green lines are the data for the untreated and GEM‐treated groups, respectively.

Article Snippet: Three human pancreatic cancer cell lines (AsPC‐1, CFPAC‐1, and PANC10.05) were purchased from the ATCC.

Techniques: Cell Culture, Western Blot, Control, Real-time Polymerase Chain Reaction, Staining, Flow Cytometry

Journal: Cancer Reports

Article Title: Senolysis of gemcitabine‐induced senescent human pancreatic cancer cells

doi: 10.1002/cnr2.2075

Figure Lengend Snippet: Combined effects of gemcitabine (GEM) and Bcl‐2 family inhibitors on cancer cells. (A) Four pancreatic cancer cell lines were cultured with GEM (0.0125 μM) for 2 days and the levels of the Bcl‐2, Bcl‐xL, and Mcl‐1 proteins were determined; β‐Actin was used as the loading control. (B) The four lines were cultured with GEM (PANC‐1 and AsPC‐1, 0.0125 μM; CFPAC‐1, 0.0008 μM; PANC10.05, 0.0032 μM) for 2 days and, after harvesting, cultured with the indicated doses of ABT‐263. After 2 days, cell viability was determined using a CCK‐8 assay. PANC‐1 and AsPC‐1 cells were cultured with GEM (0.0125 μM) for 2 days. After harvesting, cells were cultured with (C) A‐1331852 or (D) ABT‐199. After 2 days, cell viability was determined using a CCK‐8 assay. Data are means ± SD. All experiments were performed in triplicate and repeated three times.

Article Snippet: Three human pancreatic cancer cell lines (AsPC‐1, CFPAC‐1, and PANC10.05) were purchased from the ATCC.

Techniques: Cell Culture, Control, CCK-8 Assay

Journal: Cancer Reports

Article Title: Senolysis of gemcitabine‐induced senescent human pancreatic cancer cells

doi: 10.1002/cnr2.2075

Figure Lengend Snippet: Combined effects of gemcitabine (GEM) and ABT‐263 on colony formation in pancreatic cancer cells. (A) PANC‐1 and AsPC‐1 cells were cultured with GEM (0.0125 μM) and/or ABT‐263 (2 μM) for 3 days. The medium was changed and cells were cultured in the absence of any drug for a further 2 weeks; colonies were visualized using crystal violet staining. Data are means ± SD. The experiments were performed in triplicate and repeated three times. Statistical significance was evaluated using the Tukey–Kramer test (** p < 0.01). (B) Representative results are shown.

Article Snippet: Three human pancreatic cancer cell lines (AsPC‐1, CFPAC‐1, and PANC10.05) were purchased from the ATCC.

Techniques: Cell Culture, Staining